Phytopharmacy Research

Luffa acutangula L (Common name: Ridge gourd, Family: Cucurbitaceae) is a popular vegetable in India and other Asian countries. Various phytochemicals reported in L. acutangula. Chemical constituents of L. acutangula mainly include carbohydrates, carotenoids, fat, protein, phytin, amino acids (alanine, arginine, cystine, glutamic acid, glycine, hydroxyproline, leucine, serine,tryptophan), pipecolic acid, flavonoids and saponins. In order to study the compositions of the extracted constituents were analyzed by GC-MS method. The chemical compositions of leaf extracts were investigated using Perkin – Elmer GC-MS while the mass spectra of the compounds found in the leaf extracts was matched with the National Institute of Standards and Technology library. Results revealed the presence of biologically active compounds.

Syzygium species shows rich medicinal applications. The bioactive compounds from ethanolic extract of Syzygium samarangense using gas chromatography–mass spectrometry and their inhibition potential against the enzyme 3T9T were studied. The investigation focuses on the molecular simulation of bioactive compounds against the enzymes that acts as a potential drug target and supports the drug discovery process. Eighteen active compounds and their interactions with 3T9T were studied in this research work. In this study we analysed the binding affinity of 18 compounds with indicated target enzymes, through which Stigmastan-3,5-diene and Lupeol acetate is best inhibitors. Stigmastan-3,5-diene and Lupeol acetate shows highest binding energy when compared to scores obtained with the docking of remaining ligands to the respective enzymes 3T9T. And the compound Gallic acid (-5.5) shows lowest binding energies as compared to other compounds

Over one-third of the population in developing countries lack access to essential medicines. The provision of safe and effective TM/CAM therapies could become a critical tool to increase access to health care. While China, the democratic people’s republic of Korea, the republic of Korea and Vietnam have fully integrated traditional medicine into their health care systems, many countries are yet to collect and integrate standardized evidence on this type of health care. 70 countries have a national regulation on herbal medicines model. This is because medicinal products or herbs are defined differently in different countries and diverse approaches have been adopted with regard to License. The limited scientific evidence about TM/CAM’s safety and efficacy as well as other considerations.

The present study was designed to investigate the anti-hyperlipidemic activity of Viburnum opulus extract in Triton X-100 induced hyperlipidemic rats. Phytochemical Investigation revealed the presence of Alkaloid, Tannin, Saponin, Phenol in Ethanolic Extract of Viburnum opulus while only Phenol were present in Phenolic Extract of Viburnum opulus. % Yield value of Ethanolic Extract from Aerial Parts of Viburnum opulus was found to be 17%. % Administration of Triton-X 100 (100mg/kg) to all the fasted rats caused an elevation of TC, TG, VLDL and LDL and reduction in HDL levels. After 72 hours of induction of Triton X- 100 results in hyperlipidemia which is compared with normal control group, which results in significantly increased serum lipid levels in hyperlipidemic group. The change in lipid levels in group number III to VI, were comparable with group of Hyperlipidemic control (i.e Triton X-100, Group- II). The Standard group (i.e Atorvostatin group) significantly lowers the serum lipid level (P < 0.001).The results of the study clearly indicate that EEVO Extract at a dose of 500 mg/kg & 400 mg/kg significantly lowered serum lipid levels (P < 0.01). EEVO Extract at a dose of 500 mg/kg significantly lowered serum lipid levels, (P < 0.001) i.e. antihyperlipidemic activity which was found to be more effective in higher dose in triton induced hyperlipidemic models. EEVO Extracts showed a dose dependent decrease in the levels of cholesterol, Triglyceride, LDL-C and VLDL-C level. Among three groups (i.e. group number III-V), Group number-V reduced the elevated lipid levels more significantly than the other groups. (P < 0.001) Flavonoids have exhibited a variety of pharmacological activities, including the anti-atherogenesis and antioxidant effect [48]. Thus the present result strongly suggests that the hypolipidemic activity of this medicinal plant could be attributed to the presence of Tannins, Phenols, and Flavonoids in the extracts. Methods: Results: The results concluded that EEVO (500 mg/kg) has a definite anti-hyperlipidemic activity in Triton X-100 induced hyperlipidemic model and which is equipotent activity when compared with Atorvastatin treated groups. Further studies on this extract may lead to identification of the possible mechanism of action and isolation of active principle from the same. Viburnum opulus has different medicinal properties and may be able to treat diabetes and diabetic complications.When subjected to acute oral toxicity studies, it was found that Viburnum opulus is safe for use up to the dose of 1000 mg/kg. It was found to act in a dose-dependent way against Alloxan induced diabetes in rats.Reduction in the elevated blood glucose levels in diabetic rats on treatment with the extract at two different concentrations confirms that Ethanolic extract of Viburnum opulus possess anti-diabetic activity and showed an equal effect when compared to Alloxan administrated rats.

Leukemia different from most other cancers.Leukemia cells do not always form a tumor. This cancer starts in bone marrow .The bone marrow is thick ,spongy liquid inside our bones where new blood cells are made.Leukemia starts in early forms of blood cells , often white blood cells which help fight infections . When a person is infected with leukemia they may have lost the ability to fight with infections and the white blood cells lost their originality and the person may develops tumors. Leukemia cells travel in the blood and may go all over the body However, it is important to consider that the appropriate use of cell lines needs to follow established guidelines for guaranteed data reproducibility and quality , and to prevent the occurrence of detrimental events those (that are linked to cross contamination and mycoplasma contamination ).